Objective To analyze the influence from size parameters of minimally invasive vascular clamp on mechanical properties of small arteries. Methods The finite element simulation analysis on the process of minimally invasive vascular clamp clamping small arteries was performed. The influence patterns of 5 different sawtooth spacing, sawtooth heights and sawtooth lengths on mechanical properties of small arteries were studied. Results Larger sawtooth spacing led to smaller maximum equivalent stress of the clamped artery. The maximum equivalent stress of the small artery was not linear with the sawtooth height of the vascular clamp. The maximum equivalent stress of the small artery was the smallest and the vascular injury was the minimal when the swatooth height was 75 μm. The sawtooth length of the vascular clamp had an important influence on mechanical properties of clamped small arteries. The maximum equivalent stress of the artery was proportional to the sawtooth length of the vascular clamp. Conclusions The size parameters of minimally invasive vascular clamp had an important influence on mechanical properties in the process of clamping small arteries. The research findings can provide guidance for the design of the minimally invasive vascular clamp.