Bioreactor design and construct tissue engineered tendon
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    Abstract:

    Objective TO design a set of bioreactor which can construct well morphological and mechanicalperformance tissue engineered tendons. Methods According to the biomechanics and environment of tendon exist invivo, a model for compound of cells and biodegradable material was set up and a set of bioreactor which can simulatebiomechanical environment was designed. Avian tendon cell-PGA sheets were cultured in dishes during the first twoweeks. The constructs that received cyclical stretch served as experimental group, while unloaded constructs servedas control group. Specimens were harvested for gross examination, H&E staining and biomechanical test. ResultsTendon like tissue can be got under continuous and pulsatile mechanical during the cell culture in vitro. From evaluationsbetter results was proved in experimental group than control group. Conclusions mechanical model has bothcorrectness and some limits. Stress can accelerate cell matrix secret. Viscoelastic material, such as compound of celland biodegradable material, has instantaneous deformation by unvarying stress and strain increase linearly with time.Creep play an important role in collagen fibril parallel arrangement.

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. Bioreactor design and construct tissue engineered tendon[J]. Journal of medical biomechanics,2006,21(2):115-119

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