Objective A newly developed carbonated hydroxyapatite cement (CHC) was used as bone graft biomaterial and implanted into the bone. The biomechanical properties and histological features between CHC and bone were examined at different time points after implantation. Methods Mature male mongrels dogs were used, with CHC implanted into the distal femur as Detaching models' experimental group, while implantation with polymethylmethacrylate (PMMA) as control group. The biomechanical and histological properties at bone-implant interface were evaluated at different time points. Results The maximal bonding strength between CHC and bone increased along with the experimental time, up to 281N at week 16 after implantation. However, the maximal bending strength of the control group showed reverse results. Histologically, the CHC degraded gradually and replaced by new bone tissue, while the control groups showed fibrous tissue between PMMA and bone. Conclusion The CHC had excellent biocompatibility, and was able to integrated with bone directly. The bonding strength between CHC and bone increased with time post implantation.