Objective Study the difference of tensile strength, compressive strength, bending strength and twisting strength between the femoral bone and tibial bone of Qinghai bosgrunniens and lake bostaurus.Methods Crura bones taken out from 6 adult Qinghai bosgrunniens and 6 adult lake buffalos were made into standards objects as test requirement. For each test 3 samples were taken out from different oxen.With traditional mechanics method and equipment, 4 experiments on tensile strength, compressive strength,bending strength and twisting strength of the femoral bone and tibial bone have been made between bosgrunniens and bostaurus. Results For the femoral bone of Qinghai bosgrunniens tested, the tensile strength is (106.35±3.45)MPa, compressive strength is (127.60±2.65)MPa, bending strength is (225.9±4.1)MPa, and twisting strength is (53.45±1.55)MPa. For the tibial bone tested, tensile strength is (114.96±1.46)MPa, compressive strength is (184.75±3.25)MPa, bending strength (211.35±2.45)MPa, and twisting strength is (51.9±0.5)MPa. While the tibial bone of lake bostaurus the tensile strength is (128.1±11)MPa, compressive strength is (195.8±9.4)MPa, bending strength is (167.4±12.7)MPa, and twisting strength is (54.25±0.75)MPa. Conclusion Primay data of the stress of Qinghai bosgrunniens and lake bostaurus have been obtained.