Objective To study the effect of geometric parameters of micro-texture on the surface of staples in gastrointestinal microenvironment on extracellular fluid velocity and wall shear force, so as to provide theoretical references for promotion of the textured surface of staples on cell adhesion. Methods The micro-texture structure on the surface of staples was introduced into the bottom surface of flat plate flow chamber. The twodimensional ( 2D ) model of flat plate flow chamber was established by ANSYS fluent and meshed. In gastrointestinal microenvironment, the extracellular velocity and wall shear force of flat plate flow chamber with different height, side length and spacing parameters were simulated, and the data were processed and analyzed. Results The introduction of micro-texture on the surface of staples enhanced the flow velocity in plate flow chamber, and the appropriate geometric parameters of micro-texture could increase wall shear force at the microridge of micro-texture. The extracellular fluid velocity in micro-pits was low and vortices would be formed. The increase in micro-texture height, spacing and the decrease in side length could enhance the intensity of vortices in micro-pits. Conclusions The extracellular fluid velocity and wall shear force can be changed by controlling parameters of micro-texture, thus promote the adhesion of cells.