Objective Currently, the mainstream intraocular pressure( IOP) measurement devices in the market are contact and irritant, which can easily bring discomfort to patients and even lead to corneal infection during use. To solve this problem, a non-contact measurement method of IOP based on ultrasound imaging was proposed. Methods The method was verified by finite element analysis and experiment, and the simulation results of corneal deformation were obtained. A simulated eye was made and an experimental system for measuring IOP was built. A mathematical model of IOP, corneal deformation and pressure was established and its accuracy and repeatability were verified. Results The flattened diameter and vertex displacement of the cornea had a power and linear relationship with pressure under a certain IOP. Both the flattened diameter and the vertex displacement had a power relationship with IOP under constant pressure, which could be used as the index of IOP measurement. The average relative error of IOP measured by this method was smaller than 8% , the relative average deviation was smaller than 9% , and the retest reliability was greater than 0. 9. Conclusions The measurement results of the method are accurate and have a good repeatability. The results of this study provide an effective way for non-contact measurement of IOP, which has a good application prospect.