Objective To investigate the effects of unstable support surface (USS) training on balance, gait and lower limb motor function in patients with chronic stroke. Methods Twenty stroke patients were divided into two groups. Control group ( n= 10) received 12 weeks of routine rehabilitation training on the basis of conventional treatment, and experimental group ( n= 10 ) received 12 weeks of balance plate training on the basis of conventional treatment. BBS scale, Fugl-Meyer lower limb function scale, 10-meter walking speed test(10 MWT) and 6-minute walking distance test ( 6 MWD) were used to evaluate dynamic balance, walking and lower limb motor function before and after intervention. NeuroCom Balance Manager system, Qualisys 3D motion capture and analysis system and Kistler 3D force platform were used to measure the patient’s static balance, as well as their kinematics and dynamic gait data before and after intervention. Results After intervention, the differences in BBS score, Fugl-Meyer score and 6 MWD score between control group and experimental group as well as between experimental groups had statistical significance(P<0. 05), and the difference in 10 MWT score between experimental groups was statistically significant (P< 0. 05). After intervention, the difference in weigh-bearing/ squat (WB/ S) between control group and experimental group as well as between experimental groups at 90° and 60° had statistical significance (P<0. 05), and the difference in WB/ S between experimental groups at 30° and 0° was statistical significant(P<0. 05). After intervention, the differences in center of pressure (COP) symmetry and hip joint symmetry between control group and experimental group had statistical significance (P<0. 05), while the difference in symmetry of knee joint and ankle joint was not statistically significant(P>0. 05). Conclusions USS training with NeuroCom Balance Manager Balance board can effectively improve balance, gait and lower limb motor function in stroke patients.