Effects of Controllable Tensile Stress on Lumbar Disc Degeneration in Goats
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    Abstract:

    Objective: To investigate the effects of controllable tensile stress on lumbar disc degeneration in goats. Methods:Twenty-four male goats aged 6-8 months were selected to construct the IDD model by axial induction of 40N compression stress with elastic stress device. After the construction of the IDD model, they were randomly divided into 6 groups (Group A-C was the control group, and Group D-F was the experimental group). The compression stress of the IDD model was converted into tensile stress, and the traction stress of Group A-C was 0 N. The tension stress of group D-F is 40N. In 3 time periods (4 weeks, 6 weeks, 8 weeks), X-ray and MRI were used to observe the disc height and degree of degeneration (DHI, Pfirrmann grading assessment), pathological observation was made of the changes of HE staining, Ⅰ, immunohistochemical staining of type I collagen in the intervertebral disc. Results: In the control group, DHI and Pfirrmann grading showed no statistical significance with time extension (P > 0.05), while in the experimental group, DHI height increased with time extension with statistical significance (P < 0.05). Pfirrmann grading changed from low to high with time extension (V→III→IV). HE staining increased the volume of nucleus pulposus and increased nucleus pulposus cells. Immunohistochemical staining of type I collagen in nucleus pulposus changed from brown to yellow to light yellow, and 6 weeks later, the type I collagen was increased. The type II collagen staining of nucleus pulposus changed from colorless or light yellow to yellow, and the type II collagen decreased to light yellow 6 weeks later. Conclusion: The axial induced IDD can be repaired and rehydrated in a certain amount of time under a certain tensile stress, but the change of IDD will occur again if the axial tensile time is too long.

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History
  • Received:October 18,2021
  • Revised:December 21,2021
  • Adopted:December 22,2021
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