Objective The purpose of the paper is to study von Willebrand Factor(VWF) damage based on a novel Maglev Taylor-Couette blood-shearing device. Methods MagLev Taylor-Couette blood-shearing device was designed, and blood-shearing platform was built. Fresh porcine blood was tested in circulation loop for 1 h at laminar flow state. VWF damage was assessed by western blot and enzyme-linked immunosorbent assay. Results With the increase of non-physiological shear stress, a large number of high molecular weight VWF multimers were degraded into low molecular weight VWF and the activity of VWF decreased . Conclusions The VWF damage was mainly manifested by the decrease of high molecular weight VWF and the decrease of VWF activity, and VWF-Ag did not change significantly. The novel magnetic levitation Taylor-Couette blood-shearing device could quantitatively control the flow parameters (exposure time and shear stress), which can be used for blood damage research in vitro, thus providing a reference for the design and optimization of Extracorporeal membrane oxygenation(ECMO) and blood pump.