Objective: Analyze the impact of soldiers" single foot landing on the knee joint under load, and explore the relationship with muscle strength, to provide a basis for load carriage training. Methods: Using Vicon and AMTI collect kinematic and kinetic parameters of the knee joint during single foot landing from 30 cm height under 0 kg and 15 kg load from 12 soldiers. The surface electromyography of anterior and posterior thigh muscles were collected simultaneously. Then perform the knee flexion extension isokinetic force testing. Results: Compared with 0kg, the knee flexion angle and joint reaction force when landing under 15kg load increased significantly (P < 0.05), and The maximum surface electromyography of rectus femoris, medial femoral and Biceps femoris increased significantly (P < 0.05). There were no significant differences between 0 kg and 15 kg in the other indexes (P > 0.05).There was a significant negative correlation between the peak moment of knee extension and the vertical ground reaction force under 0 kg (P < 0.05), but not with the remaining measures (P > 0.05). Conclusion: Single foot landing increased the knee flexion angle and joint reaction force under 15kg load, anterior and posterior thigh muscles can relieved the ground reaction force, and muscle strength had a role in preventing landing injury.