Src/RUNX2 Modulates the Migration of Vascular Smooth Muscle Cells Induced by Cyclic Stretch
DOI:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To investigate the effect of cyclic stretch on Src and Runx related transcription factor 2 (Runx2), and their pivotal roles in migration of vascular smooth muscle cells (VSMCs). Methods Cyclic stretch of 5% (simulated normotensive physiological condition) or 15% (simulated hypertensive pathological condition) amplitude was applied to VSMCs by FX-5000T system; wWestern Blotting was used to detect the expression of RUNX2 and phosphorylation of Src in VSMCs; IPA bioinformatic software was used to analyze the potential regulatory effect of Src on RUNX2; small interfering RNA (siRNA) was transfected to decrease the expression of RUNX2; Src inhibitor-1was used to repressed Src kinase activity; wound-healing assay was applied to detect VSMC migration. Results Compared with 5% cyclic stretch, 15% cyclic stretch significantly increased RUNX2 expression in VSMCs. Under both static and 15%-cyclic-stretch conditions, VSMC migration was significantly inhibited after reducing RUNX2 expression with siRNA transfection. IPA indicated that Src kinase may be the upstream modulator of RUNX2, and western blotting validated that RUNX2 expression was significantly decreased after inhibiting Src. Furthermore, under 15% cyclic stretch condition, Src inhibitor-1 markedly repressed RUNX2 expression and VSMC migration. Conclusion High cyclic stretch increased phosphorylation of Src kinase and expression of RUNX2, which subsequently induced VSMC abnormal migration. Exploring the mechanobiological mechanism of VSMC migration regulated by cyclic stretch may contribute to further revealing the mechanism of vascular physiological homeostasis and vascular pathological remodeling. In addition, it also may provide new perspective for the translational research of vascular remodeling upon hypertension.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:April 22,2021
  • Revised:May 31,2021
  • Adopted:June 02,2021
  • Online:
  • Published: