Purpose To investigate the knee kinematics characteristics, ground reaction force (GRF) characteristics, and the stress state of cartilage and meniscus in the process of lateral incisions with different angles. Method Vicon and dynamometer were used to collect kinematics data and GRF of 14 subjects in 45 °, 90 ° and 135 ° cutting angle respectively under the expected conditions. The knee joint reaction force was obtained through the inverse dynamics calculation of visual3d. Based on the three-dimensional finite element model of knee joint, the contact process of three kinds of lateral cutting angles was simulated. Results Under the expected conditions, there were significant differences in knee joint kinematics characteristics among the three kinds of cutting angles during touchdown(p＜0.001), the degree of knee flexion increases with the increase of lateral cutting angle; The vGRF decreased significantly with the increase of lateral cutting angle (p＜0.001), hGRF showed the opposite trend; Among the three kinds of lateral cutting angles, the peak contact stress of patellar cartilage and femoral cartilage was larger at 90 ° cutting angle (6.36MPa；8.22MPa), the peak value of principal stress at ACL contact point was also larger at 90 ° cutting angle(17.47MPa), the second was 135 ° and 45 ° respectively; the peak contact stress of lateral femoral cartilage was greater than that of medial femoral cartilage at three cutting angles. Conclusion It is suggested that the risk of knee joint injury is higher in 90 ° cutting angles, and the stress state of knee joint in 135 ° cutting angles is better than that in 90 ° cutting angles, and the risk of knee joint injury does not increase with the increase of cutting angle under the expected conditions.