In osteocytes, screening of mechanoresponsive MicroRNAs which target IGF-1、NOS、FGF23 and sclerostin
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Q813.1

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    Abstract:

    Objective Stimulated by mechanical loading, osteocytes influence bone metabolism and bone formation through their secretory factor, so that this study aimed to select secretory factor-related, mechanoresponsive microRNA (miRNA) of osteocytes. Methods After mechanical tensile strain of 2500 microstrain (με) at 0.5 Hz was applied to osteocytes and osteoblasts cultured in vitro respectively, using miRNA chip, the differentially expressed miRNAs only in the osteocytes were screened out. Through bioinformatics technology, in these differentially expressed miRNAs, the target genes of which were secretory factors including insulin-like growth factor-1(IGF-1), nitric oxide synthesase (NOS), fibroblast growth factor 23 (FGF23) and sclerostin (SOST) were further selected. Then the selected miRNAs were compared with the biochip detected, differentially expressed miRNAs in femur bone of mice which were trained on treadmill and four of these miRNAs were randomly selected for quantitative PCR verification. Results In the 77 differentially expressed miRNAs only in the mechanically strained osteocytes in vitro, 22 miRNAs whose target genes are the 4 secreted factors mentioned above , were screened out. Moreover, 11 in the 22 miRNAs were differentially expressed in femur bone of the treadmill trained mice at the same trend as in osteocytes in vitro, and the randomly choiced miR-361-3p, miR-3082-5p, miR-6348 and miR-706 were confirmed to be differentially expressed at the same trend in femur bone and osteocytes. Conclusions The study indicated that these mechanoresponsive miRNAs differentially expressed only in osteocytes, probably influence osteoblastic differentiation or bone metabolism through regulating secretory factors.

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History
  • Received:October 02,2020
  • Revised:December 19,2020
  • Adopted:December 22,2020
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