Osteocytes are the main mechanical sensory and transductive cells of bone tissue. They connect with each other through many synaptic structures to form a huge regulatory network of bone steady-state cells, which are connected with osteoblasts, osteoclasts and other bone matrix surface cells. Osteocytes regulate bone metabolism and maintain bone regeneration by affecting osteoblast bone formation and osteoclast bone resorption through paracrine pathway. This paper focuses on the effects of some signal molecules or protein factors secreted or released by osteocytes after mechanical stimulation on the growth and differentiation of osteoblasts and osteoclasts. In this review, we discuss recent advances investigating how mechanically stimulated osteocytes communicate with osteoblasts and osteoclasts, providing a new ideas for the study of osteocytes biomechanics.