[Abstract] Objective To investigate the effect of Incremental training on the adenylate-activated protein kinase (AMPK) phosphorylation of skeletal muscle satellite cells in aged mice. Methods A total of 24 17-month-old male C57BL/6 mice and 12 6-week-old young male C57BL/6 mice were selected. The aged mice were randomly divided into a control group and anIncremental training group, with 12 in each group, Cage rearing. The mice in the exercise group lasted for 6 weeks. The mice in the control group lived normally in the cages without exercise. Samples were taken 24 hours after the last exercise. CD45- / CD31- / Sca1- / VCAM (CD106) + cells were isolated by flow cytometry sorting. Myogenic molecules Myod was used to the sorted cells. The cells were also cultured and induced to differentiate. immunohistochemistry combined with Western blot to detect p-AMPK levels in muscle satellite cells; Results compared with the old control group, The level of p-AMPK in young control group satellite cells was significantly increased, and the level of p-AMPK in the agedIncremental training group was significantly increased. Conclusion Endurance xercise can increase the level of AMPK phosphorylation in skeletal muscle of aged mice and improve the energy metabolism of skeletal muscle in elderly mice.