Orthopedic sutures are vital medical devices in repair surgeries of tendons and ligaments. Properties especially mechanical properties of orthopedic sutures have great impacts on the effects of tendon/ligament repairs. In this paper, clinically used orthopedic sutures including absorbable sutures and non-absorbable sutures were summarized firstly, by exploring literature about orthopedic sutures, and the mostly used orthopedic sutures are non-absorbable sutures. Then, based on the particularity of the repaired sites of orthopedic sutures, the in vitro mechanical test devices and evaluation method of orthopedic sutures were reviewed, mainly including the mechanical properties of sutures, suture-tissue, and suture-anchor. In addition, the characteristics of different evaluation method or test devices were compared. It was still difficult to evaluate the mechanical properties of orthopedic sutures due to the lack of instruments and test criteria.