The mechanobiological mechanism in vascular homeostasis and vascular remodeling is one of the most important areas in stress-growth research, which is still unclear. Proteomics analysis, which is a high-throughput and systemic technic, is recently combined with biomechanics, bioinformatics and traditional molecular biology, and applied to demonstrate the mechanism of vascular remodeling induced by different kinds of mechanical stresses. These multidisciplinary and integrated technologies give new insights into understanding the mechanobiological mechanism of vascular remodeling and provide novel potential targets of clinical therapy on cardiovascular diseases. During recent years, the Institute of Mechanobiology & Medical Engineering of Shanghai Jiao Tong University has launched systematic researches with 3 steps: phenomenon exploration with mechanobiological experiments, bioinformatics analysis, and biological and experimental verifications, which established a potential mechanotransduction networks and more than 60 kinds of the novel mechanoresponsive molecules as well. Further researches were performed to demonstrate the role of these molecules in regulation of cellular functions under different kinds of mechanical stimuli. This paper reviews the recent progresses in vascular proteomics and the relative researches on mechanobiology. Researches based on mechanics-proteomics technics may contribute to the understanding of the pathogenesis of cardiovascular diseases, and provide novel therapeutic targets for vascular remodeling during hypertension and atherosclerosis.