High flexion of the knee is very common for some specifically occupations and daily activities in many Asian and Mid-East countries. The major ligaments, including anterior cruciate ligament, posterior cruciate ligament, medial collateral ligament, lateral collateral ligament and joint capsule, are the most important static structures with function to maintain stability of the knee. Therefore, a thorough understanding on biomechanics and kinematics of the major knee ligaments at high flexion angles is significant in treatment of soft tissue release during total knee arthroplasty, anatomical cruciate ligament reconstruction and rehabilitation of knee disorders. In this paper, the research methods on knee biomechanics in recent years, and the biomechanical properties of the static knee structure from stretching to high flexion are reviewed. The further aim of the research direction in soft tissue balance during total knee arthroplasty, repair and reconstruction of knee ligaments, postoperative rehabilitation after knee injuries in the future are predicted.