Objective To make a quantitative research on the vertical force homogeneity of Yizhichan manipulation and look for the quantitative index and reference value which can describe the force homogeneity of Yizhichan manipulation effectively. Methods After the force homogeneity was described mathematically, the vertical force signals from Yizhichan manipulation operated by 5 experts, 5 skilled doctors and 5 beginners were measured and analyzed, respectively. Results The period homogeneities and the waveform homogeneities were obtained. No significant differences were found in the period homogeneities among the three groups, but significant differences appeared in waveform homogeneities between the beginner and expert group or the beginner and skilled doctor group. Conclusions The waveform homogeneities are more suitable for evaluating the vertical force homogeneity of Yizhichan manipulation by different operators, and 0.927 can be used as a reference value to evaluate the vertical force homogeneity of the operator.