Objective To develop a set of loading device that can simulate the spinal movement in vitro so as to carry out the biomechanical experiment on human spine. Methods Based on the principle of bearing, the rotary locking device was designed and fixed on the loading plate, which was rotated to the position for testing and then locked by the bolt before loading. And then, with the auto-loading power provided by the universal testing machine, the pure moment of flexion/extension, left/right bending and left/right axial rotation were applied on the spine specimen to simulate the spinal movement in vivo. Finally, the position of the spine specimen before/after loading was measured by the 3D scanner. With the loading device, the range of motion under these six loading conditions for six fresh (one-year age) porcine cervical spines (C2-C6) was tested, and precision of the loading device as well as error analysis were testified by experiments. Results A set of experimental device for the three-dimensional movement measuring for human spine was developed. Data of neutral zone and range of motion for the porcine cervical spine in six directions were acquired with the total measurement error being less than 3.5％. Conclusions The delicate design of this loading device could simulate the spinal motion in vitro and thus achieve the rapid loading of the human spine. This is an inexpensive, simple and practical device, which can significantly increase the test efficiency and has great application value in loading on the spine in vitro.