Objective To measure the iris elastic modulus. Method Using the selfdesigned device, the information of in vitro holistic iris deformation of rabbits were obtained with the loadpressure increasing gradually from 0 Pa to 600 Pa, and the heights of such iris were computed. The corresponding model based on experimental data was established and analyzed by ANSYS finite element software, and the iris elastic modulus was computed by a reverse method. Results The elastic modulus is 6.1 kPa at 100 Pa load pressure. ConclusionsThe iris material can be considered as linear elastic model when the pressure difference between the anterior chamber and posterior chamber is low.