Objective To analyse the influence from the change of blood flow at T-bifurcation vessel on thrombus formation from hydrodynamics perspectives, which mainly considers blood velocities, branch vessel diameters, T-bifurcation angles and blood viscosities. Combined with pertinent medical cases, the hypothesis in medicine about thrombus formation here is studied and validated from multidisciplinary angle. Method The geometry model of T-bifurcation artery is created to numerically analyse the flow field in vessel and the influence of different kinds of blood flow on thrombus formation. Results There are two "larger-regional" vortexes in the main and branch vessel near bifurcation. In addition, downstream the bifurcation joint there is also a smaller region, where local velocity is low and shearing stress appears to abruptly increase. This is in favor of thrombus, so to be "location apt to thrombus". Conclusions Thrombus formation in "location apt to thrombus" also has close relationship with branch vessel diameter, blood velocity, bifurcation angle and blood viscosity.