Objective Chitosan has a potential use in tissue engineered blood vessel and nerve regeneration conduit, but it degrades slowly.This research aims at minishing the chitosan conduit wall thickness with its fundamental mechanical property remained so as to shorten its degradation time.Methods Chitosan conduits were manufactured by adding some glycerol and PEG to the original solution and then using immersing-filtering methods.Effects of different molding parameters on chitosan conduit mechanical property were studied.Results Adding appropriate glycerol to original solution was of benefit to improve chitosan conduit's broken stress and broken elongation, but having no apparent effect on its Yang's modulus;the conduit's broken stress, broken elongation and Yang's modulus made of 2% chitosan were higher than that of 2.5% chitosan;with twice immersion-dryness, the conduit's broken stress, broken elongation and Yang's modulus were higher than that of thrice.Conclusion Chitosan conduit's mechanical property could be influenced by the original glycerol, the chitosan content and repeat times, and the best conduit wall thickness could be achieved by optimizing molding parameters.