Objective To explore the relation between the biochemical stimulus and big deformation during cytokinesis in eukaryotes. Methods Basis on many experiment data, A new model is proposed in this study to simulate the entire process by coupling the biochemical stimulus with the mechanical actions based on Zinemanas and Nir ’s hydrodynamic model. There were three assumptions in this model, ie. the movements of phospholipid proteins are driven by gradients of biochemical stimulus on the membrane surface, the local assembly of actin and myosin filament lies on the amount of phospholipid proteins at the same location, and the surface tension includes membrane tensions due to both the passive deformation of membrane and the active contraction of actin filament which is determined by microfilaments redistribution and rearrangement. Results These calculated results from this model showed that the contractile ring formed on early, microfilaments and the cell deformation dynamical varied during cytokinesis. Conclusion The model demonstrated that the reorientation of phospholipid proteins and the redistribution and reorientation of microfilaments may play a crucial role in cell division. This model may better represent the cytokinesis process by the introduction of biochemical stimulus.