Objective Quantifying the contribution of the non-specific interactions to the specific selection-ligand bidings. Methods We used optical tweezers to quantify the non-specific interaction dependence of bond rupture forces on loading rates and of bond lifetimes on applied forces by measuring the adhesions between two BSA (Bovine serum albumin)-coated glass beads. Results The results indicated that the rupture force dependence for non-specific interactions under lower loading rates presented from the similar transition phase as that for specific selectin-ligand bindings. While the differences in rupture force between non-specific and specific interactions became more and more evident with increase of loading rates, the increase rate of rupture force for non-specific interactions was much lower than that for selectin-ligand bindings. Under the same external force, the mean lifetime for non-specific interactions was lower than that for specific bindings. Lifetime for non-specific interactions decreased slightly with increase of applied forces, and no transition phases between slip and catch bonds were found. Conclusion This work provides the bases for quantitatively evaluating the effect of non-specific interactions in measuring selectin-ligand bindings.