Objective To investigate the adjustive tendency of cardiomyocytes’ morphological changes at low temperature. Method Compared the morphological changes of mcardiomyotytes cultured in low temperature (4 oC) with which cultured in normal temperature(37 oC), and detected cells’ interactions among cell colonies with inverted phase contrast microscope. Data were analyzed by imagine tool and mathematical statistic principles. Result (1) At the initial stages of low temperature culturing, cells assume obvious contraction with cell-cell clearance among cultured confluent cells and the orientation of clearances consistent with cell’s long axis; (2) at metaphase, present rounded or elliptical holes among the confluent cardiomyotytes; (3) at anaphase, the morphology, size and distribution of tensional holes tend to identical and cells turn to shank shape. Conclusion (1) low temperature may induce cells contract, destroy the cell-cell connection and result in histopathology; (2) Through the morphological changes and the cell-cell connective form’s regulation,Cardiomyocytes maximized the stabilization of the whole structure at low temperatures.