Objective To study the effect of anisotropic hydraulic conductivity coefficient on interstitial fluid flow in solid tumor. Methods 2-D Microvascular networks model with neoplastic microvascular morphometric and topological characteristic is built based on image manipulation. The interstitial fluid flow in connected multi-domains to account for the effect of variations hydraulic conductivity coefficient was calculated by using boundary element method. Results The difference of hydraulic conductivity coefficient between horizontal and vertical has a striking effect on the distributions of interstitial fluid pressure (IFP) in the whole tumor. Different function forms of hydraulic conductivity coefficient influence the distributions of IFP in the local region of the tumor. Conclusion Effect of anisotropic hydraulic conductivity coefficient on IFP relates to both of the various range of hydraulic conductivity coefficient and the morphology of microvascular networks.