Computer-Aided Tissue Engineering (CATE) enables many novel approaches in modeling, design, and fabrication of complex tissue substitutes with enhanced functionality and improved cell-matrix interactions. Central to CATE is its bio-tissue informatics model that represents tissue biological, biomechanical, and biochemical information that serves as a central repository to interface design, simulation and tissue fabrication. This paper presents a study of applying CATE approach for the biomimetic design of bone tissue scaffold. A general CATE-based process for biomimetic modeling, anatomic reconstruction, CAD model based tissue scaffold design, quantitative computed tomography characterization, finite element analysis and freeform extruding deposition for fabrication of scaffold is presented.