Objective To investigate the effect of OGP(10-14) and its derivatives G38I and G38K on bone biomechanical characteristics of femur and lumbar vertebra in rats with ovariectomy-induced osteoporosis. Methods Fifty-six female Sprague-Dawley rats, three month old, were randomly divided into 7 groups. Rats in group 1 to 6 were bilaterally ovariectomized (OVX) and the others in group 7 were sham-operated. Group 1 to 5 received OGP(10-14), G38I, G38K, residronate and alendronate respectively by subcutaneous injection daily after surgery. Group 6 and 7 received vehicle only. The rats were killed 60 days after operation. The left femora were taken for three-point bending test and the lumbar vertebrae were taken for compression test. The data were derived and analyzed by computer. Results The maximal stress and maximal strain of lumbar vertebra in OVX group were decreased to 80% and 65% respectively compared with the sham group. The changes in spine were more significant than that in femora and implied that cancellous bone was impacted earlier than cortical bone when osteoporosis happened. After the treatment, femoral elasticity bending stress increased significantly in G38I and G38K groups compared with OVX group. Although maximal bending stress and elastic modulus of femora enhanced in treated groups, the differences were not so significant. In OGP(10-14) -treated group, the maximal stress of lumbar vertebra was 1.28-times more than that in OVX group (P<0.01) and was similar to the group with bisphosphonates. The elastic modulus of lumbar vertebra was increased to 1.35-times in OGP(10-14) group compared with OVX group. Conclusion After the treatment with OGP(10-14) and its derivatives, the anti-impact and anti-compression ability of bone were increased in osteoporotic rats while the biomechanics was improved.