Objective To measure and compare the pullout strength of newly-designed expansive pedicle screws with that of USS,Tenor,and CDH pedicle screws in calf lumber spine, and to evaluate the screw purchase of EPS screws. Methods Sixty fresh calf lumber vertebrae harvested from 12 calf lumber spine were randomly divided into 3 groups, i.e. 40 pedicles from 20 vertebrae for each group. EPS screws were randomly inserted into one side of pedicles of all vertebrae, while USS, Tenor and CDH screws were inserted into the opposite pedicles for pullout test, respectively. The maximum axial pullout strength was used for comparison. Results The maximum axial pullout strength of the EPS, USS, Tenor and CDH screws were 2658.4N±816.7N,1929.9N±484.9N,1849.8N±575.9N and 1980.9N±836.4N, respectively. The maximum axial pullout strength of EPS screws was statistically significantly greater than that of USS, Tenor and CDH screws ( P <0.01). Conclusions Results suggested that as compared with USS, Tenor and CDH screws, the expansive pedicle screws might have better fixation effects.