Objective To discuss the biomechanical mechanism of clinical representation of gluteal muscle contracture (GMC). Methods Gluteal muscle contracture strips (GMCS) and normal gluteal muscles (NGM) were made into fasciculi. Then the biomechanical character of the specimens were measured on MTS machine.Results The elastic modulus of GMCS is averaged 2.732 N/mm 2, the maximum intension of GMCS is averaged 148.32MPa, and the maximum strain of GMCS is 0.896. In NGM, the elastic modulus is averaged 0.143N/mm 2, the maximum intension is averaged 10.50 MPa, and the maximum strain is 1.434. The difference between elastic modulus and maximum strain of GMCS and those of NGM was great (P<0.05), and the difference between maximum intension of GMCS and that of NGM was significant great (P<0.01). Conclusion The intention and rigidity of GMCS increase greatly than those of NGM. This is the biomechanical foundation for GMC in clinical representation.