Objective By evaluating quantitative biomechanical comparison of four internal fixation methods for distal femoral fractures, to choose suitable clinical internal fixation method according to the experimental data. Methods The models of fracture of femoral condyle were made on 16 fresh cadaver femurs (C1 of AO classification) and fixed by supracondylar intramedullary interlocking nail, dynamic condyle screw plate, L shaped condyle plate and Ender's nail fixation respectively. To compare the four different methods by experimental stress analysis and theoretical analysis of finite element method. Results Under the same load condition, the supracondylar intramedullary interlocking nail absorbed more energy during axial loading compared with the other constructs(P<0.01). The dynamic condyle screw plate had the greatest torsional stiffness (P<0.01). There were no significant differences in torsional stiffness between GSHN and DCS. Conclusion Using supracondylar intramdullary inter locking nail and the dynamic condyle screw plate are better than the other internal fixation methods and deserve to be put into clinical application and populatization.