Objective To develop the experimental model for explosive blast under water and analysis its distribution characteristics. Methods Depending on the theoretic principle of explosive blast,the experimental model for explosive blast under water was established. The cultured endothelial cells were placed into special asepsis plastic bag to simulate the cell injury through electric exploder of 80mg. Results The reducing velocity of explosive blast in water is slower than in air.The height of the exploder in water had obvious influence on the pressure peak of explosive blast .From the same explosive source, the deeper the water,the smaller the pressure.Different covers with filter membrane have relation to the pressure by line reducing. There is obviously crimple and exfoliate of endothelial cells injury when covered by 8 membranes. Conclusion Transmission speed of explosive blast has relation to different medium, the pressure of explosive blast under water is not only related to the distance from explosive source, but also to the height of the water.