Pedicle screw fixation in osteoprotic bone is known to be poor. This study was designed to compare four methods which can improve the screw fixation stiffness. Seven cadaveric lumbar spines with osteoprosis were obtained and BMD was evaluated using a DEXA.A11 spines were separated into 35 vertebraes, and then divided into five groups randomly. The screw were inserted using one of the five methods: (1)two screw in the same vertbrae were linked with transverse plate; (2)with bone cement into screw hole; (3)with bone pieces picked into screw hole;(4)be instead by using a larger diameter screw; and (5)with simple Dick screw fixation as control. Axial pull-out force and screw displacement were measured. The results showed that: the pull-out force was increased (compared with group 5) 93% (P<0.01) in groupl, about onefold (P<0.01) in group 2, 139% (P<0.01) in group3 and 33% (P>0.05) in group 4. It is concluded that: transverse plate, bone cement, bone pieces can improve the stability of screw. But when the vertbrae with serious osteoprosis, these methods are no clinical value. There is no significant ents with serious osteoprosis.