Objective To measure mechanical properties of calcified tissues from human aortic valve by nanoindentation test. Methods Leaflets from 5 patients with aortic stenosis were collected. Elastic modulus and hardness of the calcified leaflets were obtained by nanoindentationtest. Results The elastic modulus and hardness of the calcified leaflets were (15.69±3.89) GPa and (0.59±0.15) GPa, respectively. Conclusions The elastic modulus and hardness of the calcified valve tissues can be obtained by nanoindentationtest, which provides experimental data for biomechanical research of the valve.