刘雨佳,丁皓,张迎,施春迅,聂智超,刘浩宇,陈泽华.体外内皮细胞培养装置的研制与实验研究[J].医用生物力学,2019,34(5):541-547
体外内皮细胞培养装置的研制与实验研究
The Development and Experimental Study of Endothelial Cell Culture Device in vitro
投稿时间:2018-08-31  修订日期:2018-10-27
DOI:
中文关键词:  体外培养装置  内皮细胞  血液动力学  应力
英文关键词:in vitro culture device  endothelial cell  hemodynamics  stress
基金项目:上海市自然科学基金项目(17ZR1413500)
作者单位
刘雨佳 上海理工大学 医疗器械与食品学院 
丁皓 上海健康医学院 
张迎 上海理工大学 医疗器械与食品学院 
施春迅 上海理工大学 医疗器械与食品学院 
聂智超 上海理工大学 医疗器械与食品学院 
刘浩宇 上海理工大学 医疗器械与食品学院 
陈泽华 上海健康医学院 
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中文摘要:
      目的 研制一种创新性的体外内皮细胞培养装置,即基于血液动力学环境的血管内皮细胞体外培养装置,介绍内皮细胞体外培养装置的研制与实验研究。方法 运用血液动力学理论与方法 ,在课题组现有研究基础上设计并研制内皮细胞体外动态培养系统,其流动环境中切应力、正应力和张应力同时存在。并从装置的研制背景、结构与组成、设计原理、理论基础以及实验研究5个方面详细描述该装置的研制和实验研究。结果 装置能够准确模拟正常生理水平下内皮细胞所处的力学环境,实现切应力、正应力、张应力分别在0~12 Pa、0~15.96 kPa、0~0.5 MPa范围内的精确调控。结论 装置能提供一个更接近人体生理条件的血液动力学环境,为深入探索血管内膜损伤机制提供更理想的实验环境和手段。
英文摘要:
      Objective To develop an innovative device for endothelial cell culture in vitro, namely, to develop a vascular endothelial cell culture device based on hemodynamic environment, so as to introduce the development and experimental study of endothelial cell culture device in vitro. Methods A device of dynamic culture system for endothelial cells in vitro on the basis of the existing research was designed with the theory and method of hemodynamics. The shear stress, positive stress and tensile stress existed at the same time in the flow environment. The development and experimental research of the device were described in detail from 5 aspects, such as the development background, structure and composition, design principle, theoretical basis and experimental research. Results The device could accurately simulate the hemodynamic environment of endothelial cells at normal level, with precise control of shear stress in 0-12 Pa range, positive stress in 0-15.96 kPa range, and tensile stress in 0-0.5 MPa range. Conclusions The device can provide a hemodynamic environment which is closer to the physiological conditions of human body, as well as a more ideal experimental environment and means for further exploring the mechanism of vascular intimal injury.
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