Large-conductance Ca2+-activated K+ (BKCa) channels, activated by stress, exhibit mechanosensitivity and are involved in stress-regulated cellular function. For different types of cells, they will have different expression and activity changes due to their BKCa channels responding to different stress patterns. Correspondingly, the mechanism underling channel activation behaves differently, which is activated by the elevation in Ca2+ concentration or changes in membrane bilayer and cytoskeleton. In this review, the research progress in mechanosensitivity of BKCachannels was summarized from 3 aspects, including its molecular structure basis, manifestation and stress activation mechanism.