目的 研究溶血磷脂酸（lysophosphatidic acid, LPA）对肝癌细胞MHCC97H迁移行为的影响，并探究其相关的分子机制。方法 采用Transwell法检测LPA处理后MHCC97H细胞迁移的变化情况，并通过Rho相关蛋白激酶（ROCK）抑制剂Y-27632检测ROCK信号通路在其中的作用，利用免疫荧光染色和免疫印迹法检测LPA对MHCC97细胞骨架F-actin表达的影响，通过原子力显微镜检测LPA作用后MHCC97H细胞弹性模量的变化。结果 LPA显著促进MHCC97H的迁移，ROCK抑制剂Y-27632可阻断LPA诱导的MHCC97H细胞迁移。LPA上调MHCC97H中F-actin的表达，减小MHCC97细胞的弹性模量。结论 LPA可能主要通过ROCK/F-actin通路降低肝癌细胞MHCC97H的硬度，从而促进其迁移行为。

Objective To investigate the effect of lysophosphatidic acid (LPA) on migration of hepatocellular carcinoma MHCC97H cells and its involved mechanisms. Methods Transwell was utilized to investigate the impact of LPA on cell migration of MHCC97H cells. Furthermore, the role of ROCK in the migration of MHCC97H cells through Y-27632 (a specific inhibitor of ROCK). Then, the expression of F-actin was observed with immunofluorescence staining and Western blotting. Atomic force microscopy (AFM) was employed to investigate elastic modulus of MHCC97H cells. Results LPA significantly promoted the migration of MHCC97H cells, while Y-27632 significantly blocked the migration of MHCC97H induced by LPA. Moreover, LPA up-regulated the expression of F-actin and decreased the elastic modulus of MHCC97H cells. Conclusions LPA promotes MHCC97H cell migration through decreasing the cell stiffness via ROCK/F-actin.

国家自然科学基金项目(11272365，11532004)，高等学校博士学科点专项科研基金项目（20130191110029），中央高校基本科研业务费资助项目（106112015CDJZR238807）