Objective To study the injury cases of armored biological target by bullet and its causes, and provide references for revealing the wound mechanism of armored human by bullet and the corresponding medical treatment. Methods A 60 kg live pig was selected as the biological target, and the testing physical quantity and specific location within the biological target were identified by reference to the vulnerability in the head and chest of the soldier with armor. Three rounds of 9 mm Bala Baerum pistols in 25 meter-range were shot, respectively, on the head and chest of the biological live target with armor, and the multi-mechanical parameters (acceleration, pressure, loads, etc.) that played an important role in blunt trauma of armored biological target under pistol impacts were measured. Results (1) Blunt injury to the head of the biological target by pistol generated negative pressure pulse inside the calvarium with far reaching effects, and pressure pulse appeared in the spine and carotid; (2) Blunt injury to the chest of the biological target by pistol caused high-G impact on the heart, with high pressure wave in the lungs. Conclusions The measurement results in this study provided the basis for quantitatively understanding the injury mechanism of the pistol impacted by live armored target.