Objective To evaluate and compare the differences in COSMOL articular cartilage (AC) simulation due to the application of collagen fibril reinforcement property. Methods Collagen fiber stress was modeled independently according to its orientation in AC and written into the original poro-elastic AC model. Function call was used to avoid quadric strain term. The iteration of solver was increased for better convergence. Results The initial superficial Y displacement of the reinforced model was 15 μm, which was 17.6% of the non-reinforced model. X normal strain of the reinforced model was 10% of that in the non-reinforced model, but the superficial X normal stress of the reinforced model was 10 times higher than that of the non-reinforced model. Conclusions The application of collagen fibril reinforcement property in COMSOL AC simulation is achieved, which provided the computational model and theoretical analysis for collagen fibril lesion. Lateral reinforcement of collagen fiber can constrain the vertical strain, by which enlarge AC load capacity and improve AC mechanical properties.