Research on biomechanical testing of the spine in vitro is crucial to advance understanding about the function of spine, spine injury, spinal degeneration, and the influence of implants on spinal disorders. Spinal biomechanics is mainly composed of two parts: loading methods for the spine and measurement for the spinal movement. The loading method has gone through a process from gradual loading to continuous loading. In this paper, current literatures on spinal biomechanics were studied and up-to-date loading methods for the spinal movement in vitro were summarized to provide some references and help for the future research on loading methods of spinal movement.