Objective To investigate the effect from local vibration stimulus on the total hemoglobin and oxygen hemoglobin change of biceps muscles. Methods Arm Vibration Massage Band was used by twelve volunteers(female college students) to receive the local vibration stimulus. By using the Near Infrared Spectroscopy, the total hemoglobin and oxygen hemoglobin of biceps muscles were measured at 10th minute before vibration, 10th minute during vibration and at 15th minute after vibration, respectively, to get the variation tendency at each minute. Repeated measured one-way ANOVA was used to compare the differences in the test Results. Results The total hemoglobin of biceps muscles was significantly increased at 15th minute after vibration (P<0.05), and the total oxygen hemoglobin of biceps muscles was significantly increased at 10th minute during vibration and 15th minute after vibration (P<0.05). The maximum value of the total hemoglobin and oxygen hemoglobin occurred at 5th minute during 10-minute vibration stimulus, and compared with 10th minute before vibration, the total hemoglobin and oxygen hemoglobin could continue to be higher at 15th minute after vibration with a stable tendency. Conclusions The local vibration stimulus can acutely increase the total hemoglobin and oxygen hemoglobin of biceps muscles, which can reach the maximum value with sustained vibration stimulus for at least 5 minutes.