Objective To compare the effects of location and orientation training on the improvement of visualmotor coordination under updown reversed vision, provide reference for the preventation of space motion sickness and the design of adaptation training for sensory conflict. Method Sixteen subjects were instructed to complete the maze task wearing the updown reversing prism spectacle before and after the location training and the orientation training. Both trainings lasted 30 minutes respectively. The error rate and the executing time during the maze test were analyzed and compared. Results Both trainings significantly decreased the error rate and the executing time. In addition, the results of different training methods had significant difference, and the effects of the orientation training was better than that of the location training under up-down reversed vision. Conclusions Under the sensory conflict environment arising from the visual change, the adaptation induced by some task training could also be of benefit to some other task training so as to improve the ability of visual-motor coordination and the adaption to the sensory conflict. However, different trainings could have various degrees of improvement.