Objective To explore the method in construction of the arterial network model of female rabbit genitourinary system in application of different concentrations of perfusion fluid and different ways of infusion, laying foundation for the construction of the arterial network of digital human genitourinary system. Methods The three different concentrations of chlorinated poly vinyl chloride , that were 20%, 10% and 8% respectively, were prepared for perfusion fluid to construct the female rabbit genitourinary arterial network system through the abdominal aorta (group A) and abdominal aorta and bilateral femoral artery(group B) which were two ways of infusion, and then to analyze the expression of the cast specimens’ blood vessels . Results The arterial vascular network models of female rabbit genitourinary system ,which were injected with different concentrations of fillers and through different ways of infusions, showed a significant difference in performance: (1)the model of high concentration group resulted in obvious interrupted branches and uneven distribution. It only showed the mainly arteries but for the small artery branch, imperfectly.(2) the model of middle concentration group showed the mainly arterial course with no significant fault branches; and the distribution of vascular network is full and clear , (3) the model of low concentration group showed the mainly arteries clearly but too more periphery ones and some branches had gathered together . Conclusion The model constructed with the 10% vinyl chloride infusion liquid and through the abdominal aorta and bilateral femoral arteries simultaneously, could clearly display the full arterial vascular network.