Objective To investigate effects of dosing regiment on drug delivery in solid tumor via Lumped parameter modeling, and to validate them with experiment on rats. Methods Lumped parameter Models of pharmacokinetic and of drug delivery in tumor were developed, to simulate time courses of average drug concentration (tC) in tumor interstitium with two types of injection-methods (single-shot and trio-shot). The same performance was also made in rats experiment with two groups by given HCPT. Result It showed by lumped parameter modeling that trio-shot method is more efficacious than single-shot method and also validated by the experimental data from the rats test.C onclusion Trio- shot injection is more effective than single-shot injection. The present lumped-parameter model is quantitatively competent for the effect of different drug delivery on solid tumor.