Objective To find out accurate resonance frequency and vibration characteristic of mouse liver in low frequency as the research base for resonance trauma and its reaction on organs. Methods Seventeen male mice were anaesthetized in abdominal cavity with 1.5% sodium pentobarbital and their livers were pasted with sensors. Nine of them were fastened on vibration platform to be sweeping-frequency vibrated. The frequency which is corresponding to the biggest amplitude is the resonance frequency. The other eight mice were fastened on the platform hung horizontally by rubber band. To hammer the veneer from the down to the top, and the biggest value of frequency spectrum is the resonance frequency. Results The resonance frequency of mouse liver in chest-back direction is 3Hz by the method of vibration from 2Hz to 49Hz, and the amplitude of vibration from 2Hz to 7Hz is higher than 50% of maximum amplitude. And the result obtained by hammer method is also 3Hz. Conclusion Both the vibration method and hammer method can accurately measure resonance frequency of mouse liver, and the method can also be used to measure resonance frequency of mouse’s other organs and other animals organs. The vibration method can measure vibration characteristic of a frequency range, but the hammer method can only measure the vibration characteristic near the range of resonance frequency.