目的定性观察体外培养的人眼睫状肌细胞流体阻力的稳定性,探讨作为体外实验模型的可行性。方法培养的第4代人眼睫状肌细胞传代在Transwell半透膜上,按培养8、14、20d分为三组共15份,在细胞层上下造成7mm液面差异,观察上层液体渗透至与下层液面持平所需时间。结果除第8天组不够稳定外,另两组有60%Transwell的液体流速的误差在15%以内,80%以上的误差在30%以内。结论该模型对流体的阻力是基本稳定的。

Objective To observe the stability of liquid resistance of cultured human ciliary muscle cells. Methods To observe the time for 7mm high liquid to penetrate through 3 groups of 8、14、20 days cultured 4 passage human ciliary muscle cells on Transwell (5 in each group). Results In groups of 14、20 days cells, the differences of penetrating velocity are within 15% among 60% Transwell、within 30% among 80% Transwell. Conclusion The resistance of cultured human ciliary muscle cells to liquid is almost stable.

国家自然科学基金(10472005); 北京市自然科学基金(3052009)