The utilization of computer-aided technologies in tissue engineering has evolved in the development of a new field of Computer-Aided Tissue Engineering. This article reviews recent development and application of enabling computer technology, imaging technology, computer-aided design and manufacturing, and rapid prototyping technology in tissue engineering, particularly, in computer-aided tissue anatomical modeling, 3D anatomy visualization and 3D reconstruction, CAD-based anatomical modeling, computer-aided tissue classification, computer-aided tissue implantation and prototype modeling assisted surgical planning and reconstruction.