Objective To study the change of local biomechanical property after treatment of femoral head necrosis with BMP-2 gene therapy. Methods 11 goats with 22 sides were divided into 4 groups: Ⅰnormal group (n=6 ), Ⅱ untreated group (n=6 ), Ⅲ β-gal group (n=5), Ⅳ BMP-2 group (n=5). TCP (β-tricalcium phosphate) loaded with Adv-βgal or Adv-hBMP-2 gene transfected MSCs was implanted into femoral head at 3rd week after establishment of animal models of femoral head necrosis. All goats were killed at 16th week after treatment. Sub?-chondral bone and cancellous bone were harvested to test its biomechanical property. Results The maximum stiffness and elastic modulus of cancellous bone in BMP-2 group were (38.35±4.16) MPa and (86.85±7.28) MPa, respectively. The maximum stiffness and elastic modulus of sub-chondral bone in BMP-2 group were (80.76±5.32) MPa and (126.65±10.88) MPa, respectively. The parameters in BMP-2 group are higher than that both in β-gal group and untreated group(P<0.05), but close to normal group. Conclusion The BMP-2 Gene transfected autologous bone marrow-derived mesenchymal stem cells can accelerate the repair of femoral head necrosis and prevent the collapse of femoral head.