Biomechanics of ligament of knee joint is a hotspot in recent years. Mechanical stimulus plays an important role in modulating ligament functions and in the healing and reconstruction of ligament. This paper has reviewed some recently advance focusing on the structure, features of mechanics and biochemistry, healing and reconstruction, effect of stress applying and immobilization of ligaments, and also on the cell differentiation by stress and contrast study between anterior cruciate ligament(ACL)and medial collateral ligament (MCL)